Abstract
RNA nanotechnology is able to take advantage of the modularity of RNA to build a wide variety of structures and functional devices from a common set of structural modules. The RNA origami architecture harnesses the property of RNA to fold as it is being enzymatically synthesized by the RNA polymerase and enables the design of single-stranded devices that integrate multiple structural and functional RNA motifs. Here, we provide detailed procedures on how to design and characterize RNA origami structures. The process is illustrated by two examples: one that forms lattices and another example that acts as biosensors.
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Acknowledgment
This work was supported by the European Union’s Horizon 2020 Research and Innovation Program, as part of the Interactive Training Network, DNA Robotics, under the Marie Sklodowska-Curie grant agreement n° 765703.
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Vallina, N.S., Geary, C., Jepsen, M., Andersen, E.S. (2023). Computer-Aided Design and Production of RNA Origami as Protein Scaffolds and Biosensors. In: Valero, J. (eds) DNA and RNA Origami. Methods in Molecular Biology, vol 2639. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3028-0_3
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DOI: https://doi.org/10.1007/978-1-0716-3028-0_3
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