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Amplifluor-Based SNP Genotyping

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Plant Genotyping

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2638))

Abstract

Amplifluor, a genotyping system used to analyze single nucleotide polymorphisms (SNPs), is supplied by Merck-Millipore. Amplifluor is based on polymerase chain reaction (PCR) with two competing allele-specific primers and a SNP specific common reverse primer. Sequence information flanking SNP of interest and fluorescent plate reader for end-point measurement or qPCR machine for real time measurement are required for the execution of the Amplifluor assay. In this chapter, the principle and working protocol of the Amplifluor assay based on end-point fluorescence detection of SNP allele is presented with an example.

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Authors and Affiliations

  1. ICAR-Indian Institute of Oilseeds Research, Hyderabad, India

    Manmode Darpan Mohanrao, Senapathy Senthilvel, Yarabapani Rushwanth Reddy, Chippa Anil Kumar & Palchamy Kadirvel

Authors
  1. Manmode Darpan Mohanrao
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  2. Senapathy Senthilvel
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  3. Yarabapani Rushwanth Reddy
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  4. Chippa Anil Kumar
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  5. Palchamy Kadirvel
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Corresponding author

Correspondence to Palchamy Kadirvel .

Editor information

Editors and Affiliations

  1. College of Science and Engineering, Biological Sciences, Flinders University, Adelaide, SA, Australia

    Yuri Shavrukov

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Mohanrao, M.D., Senthilvel, S., Reddy, Y.R., Kumar, C.A., Kadirvel, P. (2023). Amplifluor-Based SNP Genotyping. In: Shavrukov, Y. (eds) Plant Genotyping. Methods in Molecular Biology, vol 2638. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3024-2_13

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  • DOI: https://doi.org/10.1007/978-1-0716-3024-2_13

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3023-5

  • Online ISBN: 978-1-0716-3024-2

  • eBook Packages: Springer Protocols

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