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FlowCam 8400 and FlowCam Cyano Phytoplankton Classification and Viability Staining by Imaging Flow Cytometry

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Spectral and Imaging Cytometry

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2635))

Abstract

This chapter provides a protocol for a detailed evaluation of phytoplankton and nuisance cyanobacteria with the FlowCam 8400 and the FlowCam Cyano. The chapter includes (i) detailed description of the quality control of fluorescent mode of the FlowCam, (ii) detailing methods for discriminating nuisance cyanobacteria using the FlowCam Cyano, how to set up libraries and classification routines for commonly used classification reports, and (iii) detailing methods for viability staining to quantify LIVE versus DEAD phytoplankton using the FlowCam 8400.

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Acknowledgements

Support for this work was provided by Yokogawa Fluid Imaging Technologies. The authors would like to thank Frances Buerkens, Savannah Judge, Sarah Isakson, and Harry Nelson from Yokogawa Fluid Imaging Technologies for providing careful review of the manuscript.

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Authors and Affiliations

  1. Yokogawa Fluid Imaging Technologies, Scarborough, ME, USA

    Kathryn H. Roache-Johnson & Nicole R. Stephens

Authors
  1. Kathryn H. Roache-Johnson
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  2. Nicole R. Stephens
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Corresponding author

Correspondence to Kathryn H. Roache-Johnson .

Editor information

Editors and Affiliations

  1. Department of Biology, School of Sciences and Humanities, Nazarbayev University, Astana, Kazakhstan

    Natasha S. Barteneva

  2. Department of Biology, School of Sciences and Humanities, Nazarbayev University, Astana, Kazakhstan

    Ivan A. Vorobjev

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Roache-Johnson, K.H., Stephens, N.R. (2023). FlowCam 8400 and FlowCam Cyano Phytoplankton Classification and Viability Staining by Imaging Flow Cytometry. In: Barteneva, N.S., Vorobjev, I.A. (eds) Spectral and Imaging Cytometry. Methods in Molecular Biology, vol 2635. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3020-4_13

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  • DOI: https://doi.org/10.1007/978-1-0716-3020-4_13

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3019-8

  • Online ISBN: 978-1-0716-3020-4

  • eBook Packages: Springer Protocols

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