Abstract
In this chapter, we present a bidirectional promoter library toolbox to evaluate fast and efficiently the optimal conditions for the balanced co-expression of two target genes. As a proof-of-concept, we demonstrate the co-expression of CYP505x and the GroEL/ES complex, which resulted in noticeably elevated enzyme activity with one of the de-novo-designed promoters of the library. The new toolbox offers a straightforward one-pot cloning approach and is highly modular. As such, the method presented here should be of great interest to any gene co-expression study.
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Jodlbauer, J., Rieder, L., Glieder, A., Wiltschi, B. (2023). Bidirectional Promoter Libraries Enable the Balanced Co-expression of Two Target Genes in E. coli. In: Kopp, J., Spadiut, O. (eds) Inclusion Bodies. Methods in Molecular Biology, vol 2617. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2930-7_5
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DOI: https://doi.org/10.1007/978-1-0716-2930-7_5
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