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Refolding of Proteins Expressed as Inclusion Bodies in E. coli

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Inclusion Bodies

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2617))

Abstract

Microbial-based biotherapeutics that are produced in Escherichia coli (E. coli) can be generated intracellularly in the form of inclusion bodies (IBs) or in soluble active form in periplasmic space or extracellularly. Overexpression of these biotherapeutics in E. coli leads to formation of insoluble aggregates called inclusion bodies. These IBs contain misfolded and inactive form of proteins which need to be refolded to obtain a functionally active form of proteins. Here, we discuss refolding of E. coli-based recombinant human granulocyte colony-stimulating factor (GCSF), expressed as IBs, and highlight some of the key features associated with the refolding kinetic reaction.

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Correspondence to Anurag S. Rathore .

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Sharma, R., Anupa, A., Rathore, A.S. (2023). Refolding of Proteins Expressed as Inclusion Bodies in E. coli. In: Kopp, J., Spadiut, O. (eds) Inclusion Bodies. Methods in Molecular Biology, vol 2617. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2930-7_14

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  • DOI: https://doi.org/10.1007/978-1-0716-2930-7_14

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2929-1

  • Online ISBN: 978-1-0716-2930-7

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