Abstract
Mitochondria are equipped with their own DNA (mtDNA), which is packed into structures termed nucleoids . While nucleoids can be visualized in situ by fluorescence microscopy , the advent of super-resolution microscopy , and in particular of stimulated emission depletion (STED), has recently enabled the visualization of nucleoids at sub-diffraction resolution. Super-resolution microscopy has proved an invaluable tool for addressing fundamental questions in mitochondrial biology. In this chapter I describe how to achieve efficient labeling of mtDNA and how to quantify nucleoid diameter using an automated approach in fixed cultured cells by STED microscopy .
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Acknowledgments
STED images were acquired at the CECAD imaging facility. E.M. is supported by the Deutsche Forschungsgemeinschaft (DFG, German Research Fundation)- SFB 1218 - grant number 269925409.
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Motori, E. (2023). Measurement of Nucleoid Size Using STED Microscopy. In: Nicholls, T.J., Uhler, J.P., Falkenberg, M. (eds) Mitochondrial DNA. Methods in Molecular Biology, vol 2615. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2922-2_9
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DOI: https://doi.org/10.1007/978-1-0716-2922-2_9
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