Abstract
Two-dimensional neutral/neutral agarose gel electrophoresis (2D-AGE) has been employed for nearly two decades in the analysis of replication and maintenance processes of animal mitochondrial DNA, but the method’s potential has not been fully exploited. Here, we describe the various steps involved in this technique, from DNA isolation, to two-dimensional neutral/neutral agarose gel electrophoresis (2D-AGE), Southern hybridization and interpretation. We also provide examples of the applicability of 2D-AGE to investigate the different features of mtDNA maintenance and regulation.
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Acknowledgements
The authors would like to thank Ian Holt, Takehiro Yasukawa and Aurelio Reyes for establishing and sharing many 2D-AGE techniques for mitochondrial DNA.
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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
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Goffart, S., Pohjoismäki, J. (2023). Analysis of Mitochondrial DNA Replication by Two-Dimensional Agarose Gel Electrophoresis. In: Nicholls, T.J., Uhler, J.P., Falkenberg, M. (eds) Mitochondrial DNA. Methods in Molecular Biology, vol 2615. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2922-2_18
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DOI: https://doi.org/10.1007/978-1-0716-2922-2_18
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