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Transposable Elements pp 369–379Cite as

Genetic Knockout of TE Insertions by CRISPR-Cas9

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Part of the Methods in Molecular Biology book series (MIMB,volume 2607)

Abstract

Transposable elements (TEs) are abundant in the genome, and specific insertions may be co-opted to act as coding or noncoding functional elements. CRISPR-based genome editing technologies enable functional studies of TE insertions in cell lines. Here, we describe the use of CRISPR-Cas9 to create and validate genetic knockouts of TEs in mammalian cell lines.

Key words

  • Transposable elements
  • CRISPR
  • Mammalian cells

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References

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Author information

Authors and Affiliations

  1. BioFrontiers Institute and Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO, USA

    David M. Simpson & Edward B. Chuong

Authors
  1. David M. Simpson
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  2. Edward B. Chuong
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Corresponding author

Correspondence to Edward B. Chuong .

Editor information

Editors and Affiliations

  1. Faculty of Medicine and Dentistry, Blizard Institute, Queen Mary University of London, London, UK

    Miguel R. Branco

  2. School of Biological and Behavioural Sciences, Queen Mary University of London, London, UK

    Alexandre de Mendoza Soler

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Simpson, D.M., Chuong, E.B. (2023). Genetic Knockout of TE Insertions by CRISPR-Cas9. In: Branco, M.R., de Mendoza Soler, A. (eds) Transposable Elements. Methods in Molecular Biology, vol 2607. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2883-6_17

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  • DOI: https://doi.org/10.1007/978-1-0716-2883-6_17

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2882-9

  • Online ISBN: 978-1-0716-2883-6

  • eBook Packages: Springer Protocols

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