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Transposable Elements pp 355–368Cite as

Epigenetic Manipulation of Transposable and Repetitive Elements

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Part of the Methods in Molecular Biology book series (MIMB,volume 2607)

Abstract

This protocol describes methods to design, assemble, and validate tools for targeted activation or repression of single-copy and multi-copy genes, including repetitive and transposable elements. It uses transcription activator-like effector (TALE) technology combined with VP64 activator or Kruppel-associated box (KRAB) repressor, both of which are potent transcriptional regulators that modify the epigenetic state of endogenous DNA loci. This protocol has been successfully used to simultaneously modify expression patterns of thousands of LINE-1 transposable elements and satellite repeats, both in cell culture model systems and in preimplantation mouse embryos.

Key words

  • Transcriptional repression/activation
  • TALE technology
  • Epigenetic modifications
  • Dual luciferase assay

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Author information

Authors and Affiliations

  1. California Institute of Technology, Pasadena, CA, USA

    Joanna W. Jachowicz

  2. Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna, Austria

    Joanna W. Jachowicz

Authors
  1. Joanna W. Jachowicz
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Corresponding author

Correspondence to Joanna W. Jachowicz .

Editor information

Editors and Affiliations

  1. Faculty of Medicine and Dentistry, Blizard Institute, Queen Mary University of London, London, UK

    Miguel R. Branco

  2. School of Biological and Behavioural Sciences, Queen Mary University of London, London, UK

    Alexandre de Mendoza Soler

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Cite this protocol

Jachowicz, J.W. (2023). Epigenetic Manipulation of Transposable and Repetitive Elements. In: Branco, M.R., de Mendoza Soler, A. (eds) Transposable Elements. Methods in Molecular Biology, vol 2607. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2883-6_16

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  • DOI: https://doi.org/10.1007/978-1-0716-2883-6_16

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2882-9

  • Online ISBN: 978-1-0716-2883-6

  • eBook Packages: Springer Protocols

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