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Quantification of Protein Palmitoylation by Cysteine-SILAC

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SILAC

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2603))

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Abstract

Cysteine-SILAC enables the detection and quantification of protein S-palmitoylation, an important protein posttranslational modification. Here we describe the cell culture, protein extraction, selective enrichment, mass spectrometry, and data analysis for palmitoylated proteins from cell samples by this method.

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Acknowledgments

The work was supported, in part, by funds from the National Key Research and Development Program (2017YFA0505100 and 2016YFA0501303), the National Science Foundation of China (21335002, 31670835, and 22074020), Shanghai Projects (18ZR1402800, 14DZ2260200, and 15JC1400700), the Ph.D. Programs Foundation of Ministry of Education of China (20130071110034), and the Key Laboratory of Glycoconjugates Research Ministry of Public Health.

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Authors and Affiliations

  1. Department of Chemistry, Fudan University, Shanghai, China

    Caiyun Fang

  2. Department of Pharmaceutics, College of Medicine, Jiaxing University, Jiaxing, China

    Xiaoqin Zhang

  3. Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai, China

    Haojie Lu

Authors
  1. Caiyun Fang
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  2. Xiaoqin Zhang
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  3. Haojie Lu
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Corresponding authors

Correspondence to Caiyun Fang or Haojie Lu .

Editor information

Editors and Affiliations

  1. Analytical Chemistry Department, Faculty of Chemical Sciences, Complutense University of Madrid, Madrid, Spain

    Jose L. Luque-Garcia

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Fang, C., Zhang, X., Lu, H. (2023). Quantification of Protein Palmitoylation by Cysteine-SILAC. In: Luque-Garcia, J.L. (eds) SILAC. Methods in Molecular Biology, vol 2603. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2863-8_5

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  • DOI: https://doi.org/10.1007/978-1-0716-2863-8_5

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2862-1

  • Online ISBN: 978-1-0716-2863-8

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