Abstract
The super-SILAC approach enables the quantitative proteome profiling of highly complex samples such as biological tissues or whole organisms. In this approach, a super-SILAC mix consisting of heavy isotope-labeled cells representative of the tissue or organism to be analyzed is mixed with the unlabeled samples of interest, such that the labeled proteins act as a spike-in standard, thus allowing the relative quantification of proteins between the samples of interest. In this chapter, we thoroughly describe the protocol to carry out the super-SILAC approach using a common in vivo model such as zebrafish larvae.
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Acknowledgments
This work was funded by the Ministerio de Ciencia e Innovación through grant PID2020-114529RB-I00. AM thanks Complutense University of Madrid and Banco Santander for a pre-doctoral fellowship (CT82/80).
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Aragoneses-Cazorla, G., Machuca, A., Buendia-Nacarino, M.P., Anunciação, D.S., Garcia-Calvo, E., Luque-Garcia, J.L. (2023). Super-SILAC Quantitative Proteome Profiling of Zebrafish Larvae. In: Luque-Garcia, J.L. (eds) SILAC. Methods in Molecular Biology, vol 2603. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2863-8_16
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DOI: https://doi.org/10.1007/978-1-0716-2863-8_16
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