Abstract
Cellular protein turnover—the net result of protein synthesis and degradation—is crucial to maintain protein homeostasis and cellular function under steady-state conditions and to enable cells to remodel their proteomes upon a perturbation. In brain cells, proteins are continuously turned over at different rates depending on various factors including cell type, subcellular localization, cellular environment, and neuronal activity. Here we describe a workflow for the analysis of protein synthesis, degradation, and turnover in primary cultured rat neurons and glia using dynamic/pulsed SILAC and mass spectrometry.
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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
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Dörrbaum, A.R., Schuman, E.M., Langer, J.D. (2023). Dynamic SILAC to Determine Protein Turnover in Neurons and Glia. In: Luque-Garcia, J.L. (eds) SILAC. Methods in Molecular Biology, vol 2603. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2863-8_1
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DOI: https://doi.org/10.1007/978-1-0716-2863-8_1
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Online ISBN: 978-1-0716-2863-8
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