Abstract
Saraca asoca is a perennial tree bearing bisexual, staminate and hermaphrodite flowers which are actinomorphic and borne in axillary corymbs. Flowering in this species begins from early December and extends up to May. Fully opened flowers are collected at 5:30 a.m. in the morning and pollen was extracted. Pollen viability was observed to be maximum in newly opened flowers and decreases progressively upon closure. Viability by in vitro germination assessment was carried out by hanging drop technique using Brewbaker and Kwack medium containing 15 percent sucrose solution. Cryopreservation was carried out by immersing fresh pollen grains in the cryobiological system containing liquid nitrogen (−196 °C). Post-viability assessment was also carried out to check the fertility status of pollen grains after cryopreservation. To use the pollen over extended period, there is a need to store pollen by maintaining its viability. The present study explains the feasibility of pollen cryopreservation in Saraca asoca.
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Rajasekharan, P.E., Harsha, R. (2023). Pollen Cryopreservation Protocol For Saraca asoca (Roxb.) De Wilde. In: Rajasekharan, P., Rohini, M. (eds) Pollen Cryopreservation Protocols . Springer Protocols Handbooks. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2843-0_47
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DOI: https://doi.org/10.1007/978-1-0716-2843-0_47
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