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Detection of Endophytes by Molecular Fingerprinting Techniques

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Endophytic Microbes: Isolation, Identification, and Bioactive Potentials

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Abstract

Endophytic fungi and bacteria are ubiquitous and occur within all known plants, including a broad range of hosts in various ecosystems, and therefore play an important role in the natural environment. Million species of endophytic fungi and bacteria are present in plant tissues. Nevertheless, our recognition of endophyte diversity is limited at present. In surveys of endophyte diversity, traditional techniques, such as culture-dependent methods, have been routinely used in since long. The discovery of endophytes in natural environments, however, has been limited by traditional methodology due to some non-culturable endophytes. Molecular techniques, such as DNA fingerprinting and sequencing methods, have been successfully employed in the detection and identification of endophytes fungi, and different endophyte diversity and community composition have been documented by cultivation-dependent and molecular techniques. This chapter summarizes the use of molecular fingerprinting protocols in the study of endophytic fungi and bacteria.

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Acknowledgments

The authors are grateful to Dr. N.P. Singh, The Director of ICAR-Indian Institute of Pulses Research, Kanpur, India for constant encouragement and support to draft this protocol.

Author information

Authors and Affiliations

  1. ICAR-Indian Institute of Pulses Research, Kanpur, UP, India

    Lakshmaiah Manjunatha, Rishikesh Kumar, Raj Kumar Mishra & Bansa Singh

  2. College of Sericulture, Chintamani, University of Agricultural Sciences, Bangalore, Karnataka, India

    Gummeral Narasareddy

  3. Directorate of Plant Protection, Quarantine & Storage, New Delhi, India

    Bankapura Mariyappa Ravikumar

  4. University of Massachusetts-Cranberry Station, East Wareham, MA, USA

    Leela Saisree Uppala

  5. ICAR-National Research Centre on Pomegranate, Solapur, Maharashtra, India

    Nanjundappa Manjunatha

  6. ICAR- Indian Grassland & Fodder Research Institute, Jhansi, UP, India

    Manikyanahalli Chandrashekara Keerthi

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  1. Lakshmaiah Manjunatha
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  9. Bansa Singh
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Editor information

Editors and Affiliations

  1. Department of Life Sciences, Sri Sathya Sai University for Human ExcellenceNavanihal, Kamalapura, Kalaburagi, Karnataka, India

    A. Sankaranarayanan

  2. C.G. Bhakta Institute of Biotechnology, Uka Tarsadia University, Surat, Gujarat, India

    Natarajan Amaresan

  3. C.G. Bhakta Institute of Biotechnology, Uka Tarsadia University, Surat, Gujarat, India

    Mitesh Kumar Dwivedi

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Manjunatha, L. et al. (2023). Detection of Endophytes by Molecular Fingerprinting Techniques. In: Sankaranarayanan, A., Amaresan, N., Dwivedi, M.K. (eds) Endophytic Microbes: Isolation, Identification, and Bioactive Potentials. Springer Protocols Handbooks. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2827-0_13

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  • DOI: https://doi.org/10.1007/978-1-0716-2827-0_13

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2826-3

  • Online ISBN: 978-1-0716-2827-0

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