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Top Five Considerations When Choosing an Antibody

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Part of the Methods in Molecular Biology book series (MIMB,volume 2593)


The choice of an antibody for a protein-based research study is one of the most crucial steps in any project. Seemingly straightforward, the process is actually quite nuanced and filled with potential pitfalls. In this chapter, we will discuss five major topics that require consideration in the antibody selection process. These include overall study objectives and resources, details of both species and clonality, suitability in applications, and available detection methods. Each section will provide background information on the topic as well as specifics of antibody use in the laboratory. This chapter may be used as a guide to help vet antibody candidates for your project so you can stain with confidence.

Key words

  • Antibody
  • Validation
  • Specificity
  • Species
  • Clonality
  • Western blot
  • Immunostaining

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Correspondence to Michael D. Browning .

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Archuleta, A., Browning, M.D. (2023). Top Five Considerations When Choosing an Antibody. In: Kalyuzhny, A.E. (eds) Signal Transduction Immunohistochemistry. Methods in Molecular Biology, vol 2593. Humana, New York, NY.

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  • Print ISBN: 978-1-0716-2810-2

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