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MRI Methods for Imaging Beta-Cell Function in the Rodent Pancreas

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Type-1 Diabetes

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2592))

Abstract

The role of Zn2+ ions in proper storage of insulin in β-cell granules is well-established so when insulin is secreted from β-cells stimulated by an increase in plasma glucose, free Zn2+ ions are also released. This local increase in Zn2+ can be detected in the pancreas of rodents in real time by the use of a zinc-responsive MR contrast agent. This method offers the opportunity to monitor β-cell function longitudinally in live rodents. The methods used in our lab are fully described in this short report and some MR images of a rat pancreas showing clearly enhanced hot spots in the tail are presented.

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Acknowledgements

The authors gratefully acknowledge the financial support from the National Institutes of Health (NIH; grant DK-095416) and valuable input from Dr. Xiaodong Wen and Dr. Shanrong Zhang.

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Authors and Affiliations

  1. Advanced Imaging Research Center, University of Texas Southwestern Medical Center, Dallas, TX, USA

    Pooyan Khalighinejad, Eul Hyun Suh & A. Dean Sherry

  2. Department of Radiology, University of Texas Southwestern Medical Center, Dallas, TX, USA

    Pooyan Khalighinejad & A. Dean Sherry

  3. Department of Chemistry & Biochemistry, University of Texas at Dallas, Richardson, TX, USA

    A. Dean Sherry

Authors
  1. Pooyan Khalighinejad
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  2. Eul Hyun Suh
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  3. A. Dean Sherry
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Corresponding author

Correspondence to A. Dean Sherry .

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Editors and Affiliations

  1. Precision Health Program, Michigan State University, East Lansing, MI, USA

    Anna Moore

  2. Precision Health Program, Michigan State University, East Lansing, MI, USA

    Ping Wang

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Khalighinejad, P., Suh, E.H., Sherry, A.D. (2023). MRI Methods for Imaging Beta-Cell Function in the Rodent Pancreas. In: Moore, A., Wang, P. (eds) Type-1 Diabetes. Methods in Molecular Biology, vol 2592. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2807-2_7

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  • DOI: https://doi.org/10.1007/978-1-0716-2807-2_7

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2806-5

  • Online ISBN: 978-1-0716-2807-2

  • eBook Packages: Springer Protocols

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