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Colony Formation Assay to Test the Impact of HDACi on Leukemic Cells

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HDAC/HAT Function Assessment and Inhibitor Development

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2589))

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Abstract

One of the main characteristics of cancer is the rapid proliferation of transformed cells. Cancer therapies aim to kill such cells. Cancer clones surviving therapy can be resistant to the treatment, but they can also lose the ability to proliferate. The ability of single cells to proliferate can be monitored in vitro and can provide insights into the sensitivity of tumor cells to chemotherapeutics. The following chapter describes how clonogenic hematopoietic cell growth can be determined with the colony formation assay.

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Acknowledgments

We gratefully acknowledge that MB is funded by the German Research Foundation/Deutsche Forschungsgemeinschaft (DFG) KR2291/9-1, project number 427404172, and intramural funding. We further thank the Brigitte and Dr. Konstanze Wegener-Stiftung (Projekt 65) for funding the research of MP.

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Authors and Affiliations

  1. Institute of Toxicology, University Medical Center Mainz, Mainz, Germany

    Miriam Pons & Mandy Beyer

Authors
  1. Miriam Pons
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  2. Mandy Beyer
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Corresponding author

Correspondence to Mandy Beyer .

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Editors and Affiliations

  1. Institute of Toxicology, University Medical Center Mainz, Mainz, Germany

    Oliver H. Krämer

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Pons, M., Beyer, M. (2023). Colony Formation Assay to Test the Impact of HDACi on Leukemic Cells. In: Krämer, O.H. (eds) HDAC/HAT Function Assessment and Inhibitor Development. Methods in Molecular Biology, vol 2589. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2788-4_2

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  • DOI: https://doi.org/10.1007/978-1-0716-2788-4_2

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2787-7

  • Online ISBN: 978-1-0716-2788-4

  • eBook Packages: Springer Protocols

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