Skip to main content

Detection and Quantification of Protein Aggregates in Plants

  • Protocol
  • First Online:
Plant Proteostasis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2581))

  • 914 Accesses

Abstract

Protein quality control is an important aspect of stress recovery. It maintains protein homeostasis through a machinery of regulatory proteins such as chaperones and proteases. When the system recognizes accumulation of misfolded or aggregated proteins, the cell recruits a set of regulatory proteins to initiate protein quality control. To understand the dynamics of stress-mediated aggregate protein formation and recovery in plants, robust methods aimed at detecting and measuring such protein aggregates are needed. This will help us to deepen our understanding of protein quality control mechanisms in plants.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 249.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Similar content being viewed by others

References

  1. Gidalevitz T, Prahlad V, Morimoto RI (2011) The stress of protein misfolding: from single cells to multicellular organisms. Cold Spring Harb Perspect Biol 3. https://doi.org/10.1101/cshperspect.a009704

  2. Díaz-Villanueva JF, Díaz-Molina R, García-González V (2015) Protein folding and mechanisms of proteostasis. Int J Mol Sci 16:17193–17230. https://doi.org/10.3390/ijms160817193

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Hill SM, Hanzén S, Nyström T (2017) Restricted access: spatial sequestration of damaged proteins during stress and aging. EMBO Rep 18:377–391. https://doi.org/10.15252/embr.201643458

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Nakajima Y, Suzuki S (2013) Environmental stresses induce misfolded protein aggregation in plant cells in a microtubule-dependent manner. Int J Mol Sci 14:7771–7783. https://doi.org/10.3390/ijms14047771

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Chen B, Retzlaff M, Roos T, Frydman J (2011) Cellular strategies of protein quality control. Cold Spring Harb Perspect Biol 3:a004374. https://doi.org/10.1101/cshperspect.a004374

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Bukau B, Weissman J, Horwich A (2006) Molecular chaperones and protein quality control. Cell 125:443–451. https://doi.org/10.1016/j.cell.2006.04.014

    Article  CAS  PubMed  Google Scholar 

  7. Hill SM, Hao X, Liu B, Nyström T (2014) Life-span extension by a metacaspase in the yeast Saccharomyces cerevisiae. Science (80- ). https://doi.org/10.1126/science.1252634

  8. Hill SM, Nyström T (2015) The dual role of a yeast metacaspase: What doesn’t kill you makes you stronger. BioEssays. https://doi.org/10.1002/bies.201400208

  9. Coll NS, Smidler A, Puigvert M et al (2014) The plant metacaspase AtMC1 in pathogen-triggered programmed cell death and aging: Functional linkage with autophagy. Cell Death Differ. https://doi.org/10.1038/cdd.2014.50

  10. Sedaghatmehr M, Thirumalaikumar VP, Kamranfar I et al (2019) A regulatory role of autophagy for resetting the memory of heat stress in plants. Plant Cell Environ. https://doi.org/10.1111/pce.13426

  11. McLoughlin F, Kim M, Marshall RS et al (2019) HSP101 interacts with the proteasome and promotes the clearance of ubiquitylated protein aggregates. Plant Physiol. https://doi.org/10.1104/pp.19.00263

  12. Lema Asqui S, Vercammen D, Serrano I et al (2018) AtSERPIN1 is an inhibitor of the metacaspase AtMC1-mediated cell death and autocatalytic processing in planta. New Phytol. https://doi.org/10.1111/nph.14446

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Núria S. Coll .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

About this protocol

Check for updates. Verify currency and authenticity via CrossMark

Cite this protocol

Phukan, U.J., Stael, S., Gonçalves, A., Van Breusegem, F., Coll, N.S. (2023). Detection and Quantification of Protein Aggregates in Plants. In: Lois, L.M., Trujillo, M. (eds) Plant Proteostasis. Methods in Molecular Biology, vol 2581. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2784-6_15

Download citation

  • DOI: https://doi.org/10.1007/978-1-0716-2784-6_15

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2783-9

  • Online ISBN: 978-1-0716-2784-6

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics