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Quantitative Analysis of B-Cell Subpopulations in Bone Marrow by Flow Cytometry

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West Nile Virus

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2585))

Abstract

Flow cytometry is a technology that rapidly detects and measures physical and chemical characteristics of single cells or particles. It is a powerful tool for many areas of research, in particular, immunology, which allows for simultaneous analysis of different immune cell populations in a tissue. Here we describe the procedures to quantify and/or purify various B fractions in mouse bone marrows by flow cytometry using their signature surface markers. This method is useful to study B-cell development during steady-state or emergency hematopoiesis such as viral infections.

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Acknowledgments

This work was supported by a US National Institutes of Health research award NIAID R01AI132526 to P.W.

Conflict of Interest

The authors declare no financial or nonfinancial interest.

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Authors and Affiliations

  1. Department of Immunology, School of Medicine, UConn Health, Farmington, CT, USA

    Tingting Geng & Penghua Wang

Authors
  1. Tingting Geng
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  2. Penghua Wang
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Corresponding authors

Correspondence to Tingting Geng or Penghua Wang .

Editor information

Editors and Affiliations

  1. Department of Cell and Molecular Biology, Center for Molecular and Cellular Biosciences The University of Southern Mississippi, Hattiesburg, MS, USA

    Fengwei Bai

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Geng, T., Wang, P. (2023). Quantitative Analysis of B-Cell Subpopulations in Bone Marrow by Flow Cytometry. In: Bai, F. (eds) West Nile Virus. Methods in Molecular Biology, vol 2585. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2760-0_8

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  • DOI: https://doi.org/10.1007/978-1-0716-2760-0_8

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2759-4

  • Online ISBN: 978-1-0716-2760-0

  • eBook Packages: Springer Protocols

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