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West Nile Virus pp 33–40Cite as

Isolation of Murine Bone Marrow-Derived Neutrophils for Infection Modeling

Part of the Methods in Molecular Biology book series (MIMB,volume 2585)

Abstract

West Nile virus (WNV) is a single-stranded-RNA flavivirus that can cause neurological illnesses. The ability of WNV to cause neurological illnesses is dependent on the virus’ ability to gain access into the brain. However, the mechanisms by which WNV enters the brain are elusive, with evidence that neutrophils act as vehicles for viral delivery. To determine the role of neutrophils in WNV central nervous system delivery, modified protocols for the isolation and migration of neutrophils from mouse bone marrow for in vitro WNV infection modeling is described.

Key words

  • Bone marrow-derived neutrophils
  • West Nile virus
  • Migration
  • Osteopontin

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  • DOI: 10.1007/978-1-0716-2760-0_5
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References

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Acknowledgments

This work was supported by Embry-Riddle Aeronautical University’s start-up fund and Wessel Foundation endowment to AMP.

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Authors and Affiliations

  1. Department of Cell and Molecular Biology, University of Southern Mississippi, Hattiesburg, MS, USA

    Laurel Duty & Amber M. Paul

  2. Space Biosciences Division, NASA Ames Research Center, Moffett Field, CA, USA

    Amber M. Paul

  3. Blue Marble Space Institute of Science, Seattle, WA, USA

    Amber M. Paul

  4. Department of Human Factors and Behavioral Neurobiology, Embry-Riddle Aeronautical University, College of Arts & Sciences (COAS), Daytona Beach, FL, USA

    Amber M. Paul

Authors
  1. Laurel Duty
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  2. Amber M. Paul
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Corresponding author

Correspondence to Amber M. Paul .

Editor information

Editors and Affiliations

  1. Department of Cell and Molecular Biology, Center for Molecular and Cellular Biosciences The University of Southern Mississippi, Hattiesburg, MS, USA

    Fengwei Bai

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Duty, L., Paul, A.M. (2023). Isolation of Murine Bone Marrow-Derived Neutrophils for Infection Modeling. In: Bai, F. (eds) West Nile Virus. Methods in Molecular Biology, vol 2585. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2760-0_5

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  • DOI: https://doi.org/10.1007/978-1-0716-2760-0_5

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2759-4

  • Online ISBN: 978-1-0716-2760-0

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