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West Nile Virus pp 23–31Cite as

Isolate and Culture Mouse Primary Neurons for West Nile Virus Infection

Part of the Methods in Molecular Biology book series (MIMB,volume 2585)

Abstract

Primary neurons are very valuable cells to study the pathogenesis of neurotropic viruses, such as West Nile virus. The mouse primary neurons can be used to assess viral infection profiles and cellular immune responses to a viral infection. However, successful isolation and culture of mouse neurons can be challenging. Here, we report a step-by-step method to prepare a primary neuron culture from adult mice.

Key words

  • Mouse primary neuron
  • Culture protocol
  • Neurotrophic virus
  • West Nile virus

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  • DOI: 10.1007/978-1-0716-2760-0_4
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Acknowledgments

This work was supported in part by the National Institute of Allergy and Infectious Diseases of the National Institutes of Health R15AI135893 (F.B.).

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Authors and Affiliations

  1. Department of Cell and Molecular Biology, Center for Molecular and Cellular Biosciences, The University of Southern Mississippi, Hattiesburg, MS, USA

    Farzana Nazneen & Fengwei Bai

Authors
  1. Farzana Nazneen
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  2. Fengwei Bai
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Corresponding author

Correspondence to Fengwei Bai .

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Editors and Affiliations

  1. Department of Cell and Molecular Biology, Center for Molecular and Cellular Biosciences The University of Southern Mississippi, Hattiesburg, MS, USA

    Fengwei Bai

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Nazneen, F., Bai, F. (2023). Isolate and Culture Mouse Primary Neurons for West Nile Virus Infection. In: Bai, F. (eds) West Nile Virus. Methods in Molecular Biology, vol 2585. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2760-0_4

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  • DOI: https://doi.org/10.1007/978-1-0716-2760-0_4

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2759-4

  • Online ISBN: 978-1-0716-2760-0

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