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Purification of Bone Marrow Precursors to T Cells and ILCs

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Part of the Methods in Molecular Biology book series (MIMB,volume 2580)

Abstract

T cells and innate lymphoid cells (ILCs) share expression of many key transcription factors during development and at mature stage, resulting in striking functional similarities between these lineages. Taking into account ILC contribution is thus necessary to appreciate T cell functions during immune responses. Furthermore, understanding ILC development and functions helps to understand T cells. Here we provide methods and protocols to isolate pure populations of multipotent precursors to T cells and innate lymphoid cells (ILCs) from adult mouse bone marrow, using flow cytometric sorting. These include precursors to all lymphocytes (viz., LMPPs and ALPs) and multipotent precursors to ILCs that have been recently refined (viz., specified EILPs, committed EILPs, and ILCPs).

Key words

  • T cell development
  • ILC development
  • Multipotent precursors
  • LMPP
  • ALP
  • EILP
  • ILCP

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Correspondence to Christelle Harly .

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Kenney, D., Harly, C. (2023). Purification of Bone Marrow Precursors to T Cells and ILCs. In: Bosselut, R., Vacchio, M.S. (eds) T-Cell Development. Methods in Molecular Biology, vol 2580. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2740-2_13

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  • DOI: https://doi.org/10.1007/978-1-0716-2740-2_13

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2739-6

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