Abstract
The thymus is compartmentalized into the cortex and the medulla. Cortical and medullary thymic epithelial cells (TECs) characterize T cell-producing and T cell-selecting functions of cortical and medullary microenvironments in the thymus. Enzymatic digestion of the thymus and flow cytometric isolation of TECs and their subpopulations are useful for molecular and cellular characterization of TECs. However, the cellularity of cTECs and mTECs isolated from mouse thymus is limited. In this chapter, we describe the method for isolation of a large number of TECs using enlarged mouse thymus, which enables biochemical and proteomic analysis of TEC subpopulations.
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Acknowledgments
The authors thank Alison Jacques for reading the manuscript. The research was supported in part by the Intramural Research Program of the NIH, NCI, and EIB.
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Ohigashi, I., Matsuda-Lennikov, M., Takahama, Y. (2023). Large-Scale Isolation of Mouse Thymic Epithelial Cells. In: Bosselut, R., Vacchio, M.S. (eds) T-Cell Development. Methods in Molecular Biology, vol 2580. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2740-2_11
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DOI: https://doi.org/10.1007/978-1-0716-2740-2_11
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