Abstract
Golgi-derived microtubules constitute an asymmetrical microtubule network that drives polarized transport of vesicles to support cell polarization and directional migration. Golgi-based microtubule nucleation requires the γ-tubulin ring complex (γTuRC), the principal microtubule nucleator in animal cells. In this chapter, we present methods for detecting γTuRC components and associated proteins on the Golgi, examining Golgi-based microtubule nucleation, and measuring the microtubule-nucleating activity of isolated γTuRCs. These approaches have been demonstrated to be effective for assessing the microtubule-organizing function of the Golgi complex.
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Acknowledgments
This work was supported by grants from the Research Grants Council (General Research Fund and Theme-Based Research Scheme T13-605/18-W) and the Innovation and Technology Commission (ITCPD/17-9) of Hong Kong. F.K.C.A. was supported by the Postdoctoral Fellowship Scheme (PDFS2021-6S03) of the Research Grants Council of Hong Kong.
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Au, F.K.C., Le, K.T.D., Qi, R.Z. (2023). Detection and Analysis of Microtubule Nucleator γ-Tubulin Ring Complex. In: Wang, Y., Lupashin, V.V., Graham, T.R. (eds) Golgi. Methods in Molecular Biology, vol 2557. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2639-9_32
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DOI: https://doi.org/10.1007/978-1-0716-2639-9_32
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