Skip to main content

Polygalacturonase

  • Protocol
  • First Online:
Biotic Elicitors

Part of the book series: Springer Protocols Handbooks ((SPH))

  • 229 Accesses

Abstract

Polygalacturonase (PG) is the first cell wall-degrading enzyme synthesized by phytopathogenic fungi and bacteria. This enzyme is involved in basic and specific compatibility between microorganisms and higher plants. Polygalacturonases have several functions, such as during fruit ripening, pollen tube elongation, abscission process, and plant-pathogen interactions, which cause pathogenicity by fungal/bacterial attack. It has been significantly less explored in the field of plant defense elicitation. Therefore, the present chapter provides insights into protocols for the production, purification, and identification of PG from fungi.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Lang C, Dörnenburg H (2000) Perspectives in the biological function and the technological application of polygalacturonases. Appl Microbiol Biotechnol 53(4):366–375

    Article  CAS  Google Scholar 

  2. Federici L, Di Matteo A, Fernandez-Recio J, Tsernoglou D, Cervone F (2006) Polygalacturonase inhibiting proteins: players in plant innate immunity? Trends Plant Sci 11(2):65–70

    Article  CAS  Google Scholar 

  3. De Lorenzo G, D'Ovidio R, Cervone F (2001) The role of polygalacturonase-inhibiting proteins (PGIPs) in defense against pathogenic fungi. Annu Rev Phytopathol 39(1):313–335

    Article  Google Scholar 

  4. Boller T (1995) Chemoperception of microbial signals in plant cells. Annu Rev Plant Physiol Plant Mol Biol 46:189–214

    Article  CAS  Google Scholar 

  5. Favela-Torres E, Volke- Sepúlveda T, Viniegra-González G (2006) Production of hydrolytic depolymerising pectinases. Food Technol Biotechnol 44:221–227

    CAS  Google Scholar 

  6. Pandey A, Selvakumar P, Soccol CR, Nigam P (1999) Solid state fermentation for the production of industrial enzymes. Curr Sci 77:149–162

    CAS  Google Scholar 

  7. Pandey A, Soccol CR, Nigam P, Soccol VT (2000) Biotechnological potential of agro-industrial residues. I: sugar cane bagasse. Bioresour Technol 74:69–80

    Article  CAS  Google Scholar 

  8. Pandey A (2003) Solid-state fermentation. Biochem Eng J 13:81–84

    Article  CAS  Google Scholar 

  9. Patidar MK, Nighojkar A, Nighojkar S, Kumar A (2017) Purification and characterization of polygalacturonase produced by Aspergillus niger AN07 in solid state fermentation. Can J Biotechnol 1(1):11–18

    Article  Google Scholar 

  10. Nelson N (1944) A photometric adaptation of the Somogyi method for the determination of glucose. J Biol Chem 153(2):375–380

    Article  CAS  Google Scholar 

  11. Somogyi M (1952) Notes on sugar determination. J Biol Chem 195:19–23

    Article  CAS  Google Scholar 

  12. Lowry O, Rosebrough N, Farr AL, Randall R (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193(1):265–275

    Article  CAS  Google Scholar 

  13. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227(5259):680–685

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

About this protocol

Check for updates. Verify currency and authenticity via CrossMark

Cite this protocol

Chaudhary, K., Amin, D. (2022). Polygalacturonase. In: Amin, D., Amaresan, N., Ray, S. (eds) Biotic Elicitors. Springer Protocols Handbooks. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2601-6_20

Download citation

  • DOI: https://doi.org/10.1007/978-1-0716-2601-6_20

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2600-9

  • Online ISBN: 978-1-0716-2601-6

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics