Abstract
Polygalacturonase (PG) is the first cell wall-degrading enzyme synthesized by phytopathogenic fungi and bacteria. This enzyme is involved in basic and specific compatibility between microorganisms and higher plants. Polygalacturonases have several functions, such as during fruit ripening, pollen tube elongation, abscission process, and plant-pathogen interactions, which cause pathogenicity by fungal/bacterial attack. It has been significantly less explored in the field of plant defense elicitation. Therefore, the present chapter provides insights into protocols for the production, purification, and identification of PG from fungi.
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References
Lang C, Dörnenburg H (2000) Perspectives in the biological function and the technological application of polygalacturonases. Appl Microbiol Biotechnol 53(4):366–375
Federici L, Di Matteo A, Fernandez-Recio J, Tsernoglou D, Cervone F (2006) Polygalacturonase inhibiting proteins: players in plant innate immunity? Trends Plant Sci 11(2):65–70
De Lorenzo G, D'Ovidio R, Cervone F (2001) The role of polygalacturonase-inhibiting proteins (PGIPs) in defense against pathogenic fungi. Annu Rev Phytopathol 39(1):313–335
Boller T (1995) Chemoperception of microbial signals in plant cells. Annu Rev Plant Physiol Plant Mol Biol 46:189–214
Favela-Torres E, Volke- Sepúlveda T, Viniegra-González G (2006) Production of hydrolytic depolymerising pectinases. Food Technol Biotechnol 44:221–227
Pandey A, Selvakumar P, Soccol CR, Nigam P (1999) Solid state fermentation for the production of industrial enzymes. Curr Sci 77:149–162
Pandey A, Soccol CR, Nigam P, Soccol VT (2000) Biotechnological potential of agro-industrial residues. I: sugar cane bagasse. Bioresour Technol 74:69–80
Pandey A (2003) Solid-state fermentation. Biochem Eng J 13:81–84
Patidar MK, Nighojkar A, Nighojkar S, Kumar A (2017) Purification and characterization of polygalacturonase produced by Aspergillus niger AN07 in solid state fermentation. Can J Biotechnol 1(1):11–18
Nelson N (1944) A photometric adaptation of the Somogyi method for the determination of glucose. J Biol Chem 153(2):375–380
Somogyi M (1952) Notes on sugar determination. J Biol Chem 195:19–23
Lowry O, Rosebrough N, Farr AL, Randall R (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193(1):265–275
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227(5259):680–685
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Chaudhary, K., Amin, D. (2022). Polygalacturonase. In: Amin, D., Amaresan, N., Ray, S. (eds) Biotic Elicitors. Springer Protocols Handbooks. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2601-6_20
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DOI: https://doi.org/10.1007/978-1-0716-2601-6_20
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