Abstract
Glycoproteins are complex molecules with glycosidic substituents; a firm covalent attachment is present between sugars and proteins. Glycoproteins often exhibit different degrees of glycosylation. It is difficult to purify and characterize partly because of the variable glycosylation produced during or after biosynthesis. In agriculture, glycoproteins extracted from bacteria or fungi are potential biological agents for application in plant disease management. Glycoprotein elicitors can induce plant resistance and represent an effective alternative strategy for plant disease control compared to traditional methods. This chapter deals with standard protocols for glycoprotein production, purification, and characterization for further exploration in this field.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Klarzynski O, Descamps V, Plesse B, Yvin JC, Kloareg B, Fritig B (2003) Sulfated fucan oligosaccharides elicit defense responses in tobacco and local and systemic resistance against tobacco mosaic virus. Mol Plant-Microbe Interact 16(2):115–122
Thatcher LF, Anderson JP, Singh KB (2005) Plant defence responses: what have we learnt from Arabidopsis? Funct Plant Biol 32(1):1–19
Nürnberger T, Lipka V (2005) Non-host resistance in plants: new insights into an old phenomenon. Mol Plant Pathol 6(3):335–345
Pare PW, Farag MA, Krishnamachari V, Zhang H, Ryu CM, Kloepper JW (2005) Elicitors and priming agents initiate plant defense responses. Photosynth Res 85(2):149–159
Ebel J, Grisebach H (1988) Defense strategies of soybean against the fungus Phytophthora megasperma f. sp. glycinea: a molecular analysis. Trends Biochem Sci 13(1):23–27
Parker JE, Schulte W, Hahlbrock K, Scheel D (1991) An extracellular glycoprotein from Phytophthora megasperma f. sp. glycinea elicits phytoalexin synthesis in cultured parsley cells and protoplasts. Mol Plant-Microbe Interact 4:19–27
Baillieul F, Genetet I, Kopp M, Saindrenan P, Fritig B, Kauffmann S (1995) A new elicitor of the hypersensitive response in tobacco: a fungal glycoprotein elicits cell death, expression of defence genes, production of salicylic acid, and induction of systemic acquired resistance. Plant J 8(4):551–560
Yang X, Qiu D, Zeng H, Yuan J, Mao J (2009) Purification and characterization of a glycoprotein elicitor from Alternaria tenuissima. World J Microbiol Biotechnol 25(11):2035–2042
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227(5259):680–685
Schägger H, Von Jagow G (1987) Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal Biochem 166(2):368–379
Oakley BR, Kirsch DR, Morris NR (1980) A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels. Anal Biochem 105(1):361–363
Strömqvist M, Gruffman H (1992) Periodic acid/Schiff staining of glycoproteins immobilized on a blotting matrix. BioTechniques 13(5):744–746
Bradford MM (1976) A rapid and sensitive method for the quantization of microgram quantities of protein utilizing the principle of protein–dye binding. Anal Biochem 72:248–254
Bi Y, Liu Y, Zhang P, Sun SK, Zhang Q, Shao H (2006) Measurement of total sugar content by anthrone reagent in soybean whey waste water. Soybean Bull 3:24–25
Cordelier S, Ruffray P, Fritg B, Kauffmann S (2003) Biological and molecular comparison between localized and systemic acquired resistance induced in tobacco by Phytophthora megasperma glycoprotein elicitin. Plant Mol Biol 51:109–118
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Chaudhary, K., Amin, D., Amaresan, N. (2022). Glycoprotein. In: Amin, D., Amaresan, N., Ray, S. (eds) Biotic Elicitors. Springer Protocols Handbooks. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2601-6_19
Download citation
DOI: https://doi.org/10.1007/978-1-0716-2601-6_19
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-2600-9
Online ISBN: 978-1-0716-2601-6
eBook Packages: Springer Protocols