Skip to main content

Pharmacogenomic Screening of Drug Candidates using Patient-Specific hiPSC-Derived Cardiomyocyte High-Throughput Calcium Imaging

  • Protocol
Pharmacogenomics in Drug Discovery and Development

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2547))

Abstract

Calcium imaging is an invaluable technique to detect and characterize calcium flux in cells. The use of calcium dye provides information on the concentration and spatial distribution of calcium. Calcium imaging is a well-established technique to assess the calcium-induced calcium release mechanism in cardiomyocytes. It can also be used to characterize mutations in genes crucial for this mechanism that frequently causes arrhythmia. Here we describe a high-throughput methodology of calcium imaging that records individual calcium transients in more than 10,000 human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) in less than 30 min.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 139.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 179.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Similar content being viewed by others

References

  1. Knot HJ, Laher I, Sobie EA et al (2005) Twenty years of calcium imaging: cell physiology to dye for. Mol Interv 5:112–127

    Article  CAS  PubMed  Google Scholar 

  2. Bers DM (2002) Cardiac excitation-contraction coupling. Nature 415:198–205

    Article  CAS  PubMed  Google Scholar 

  3. Landstrom AP, Dobrev D, Wehrens XHT (2017) Calcium signaling and cardiac arrhythmias. Circ Res 120:1969–1993

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Ridgway EB, Ashley CC (1967) Calcium transients in single muscle fibers. Biochem Biophys Res Commun 29:229–234

    Article  CAS  PubMed  Google Scholar 

  5. Wier WG (1980) Calcium transients during excitation-contraction coupling in mammalian heart: aequorin signals of canine purkinje fibers. Science 207:1085–1087

    Article  CAS  PubMed  Google Scholar 

  6. Cheng H, Lederer WJ, Cannell MB (1993) Calcium sparks: elementary events underlying excitation-contraction coupling in heart muscle. Science 262:740–744

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgement

This work was supported by the National Institutes of Health (NIH) Distinguished Scholars Program (DSP) to LMR and the Division of Intramural Research (DIR) of the National Institute on Minority Health and Health Disparities (NIMHD) at NIH (1ZIAMD000016 and 1ZIAMD000018).

This work was supported by the FAPA project (PVI0122029) from Universidad de Los Andes, granted to A.V.A.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Paul W. Burridge .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

About this protocol

Cite this protocol

Blancard, M., Fetterman, K.A., Burridge, P.W. (2022). Pharmacogenomic Screening of Drug Candidates using Patient-Specific hiPSC-Derived Cardiomyocyte High-Throughput Calcium Imaging. In: Yan, Q. (eds) Pharmacogenomics in Drug Discovery and Development. Methods in Molecular Biology, vol 2547. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2573-6_10

Download citation

  • DOI: https://doi.org/10.1007/978-1-0716-2573-6_10

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2572-9

  • Online ISBN: 978-1-0716-2573-6

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics