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A Novel High-Throughput Phenotyping Hydroponic System for Nitrogen Deficiency Studies in Arabidopsis thaliana

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High-Throughput Plant Phenotyping

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2539))

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Abstract

High-throughput phenotyping enables the temporal detection of subtle changes in plant plasticity and adaptation to different conditions, such as nitrogen deficiency, in an accurate, nondestructive, and unbiased way. Here, we describe a protocol to assess the contribution of nitrogen addition or deprival using an image-based system to analyze plant phenotype. Thousands of images can be captured throughout the life cycle of Arabidopsis, and those images can be used to quantify parameters such as plant growth (area, caliper length, diameter, etc.), in planta chlorophyll fluorescence, and in planta relative water content.

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Acknowledgments

The Scanalyzer HTS was acquired with funds from the Plant Powered Production (P3) Center by NSF grant # EPS-0701890. This work was funded by NSF EPSCoR Track 2 grants # IIA-1430427 and #1736192 to AL. We thank additional funding provided by the Arkansas Biosciences Institute (ABI) and the Arkansas Research Alliance (ARA).

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Correspondence to Benjamin Babst or Argelia Lorence .

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Acosta-Gamboa, L.M., Campbell, Z.C., Gao, F., Babst, B., Lorence, A. (2022). A Novel High-Throughput Phenotyping Hydroponic System for Nitrogen Deficiency Studies in Arabidopsis thaliana. In: Lorence, A., Medina Jimenez, K. (eds) High-Throughput Plant Phenotyping. Methods in Molecular Biology, vol 2539. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2537-8_3

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  • DOI: https://doi.org/10.1007/978-1-0716-2537-8_3

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2536-1

  • Online ISBN: 978-1-0716-2537-8

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