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DNA Extraction Methods to Obtain High DNA Quality from Different Plant Tissues

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Plant Pathology


DNA extraction from plant samples is very important for a good performance of diagnostic molecular assays in phytopathology. The variety of matrices (such as leaves, roots, and twigs) requires a differentiated approach to DNA extraction. Here we describe three categories of matrices: (a) symptomatic bark/wood tissue; (b) residues of frass resulting from insect woody trophic activities, portions of the galleries produced in the wood, and tissues surrounding exit holes; and (c) leaves of different plant species. To improve the performances of diagnostic assays, we here describe DNA extraction procedures that have been optimized for each matrix type.

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Correspondence to Elisabetta Rossi .

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Rizzo, D. et al. (2022). DNA Extraction Methods to Obtain High DNA Quality from Different Plant Tissues. In: Luchi, N. (eds) Plant Pathology. Methods in Molecular Biology, vol 2536. Humana, New York, NY.

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2516-3

  • Online ISBN: 978-1-0716-2517-0

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