Abstract
Peptide mapping is a routine procedure for protein characterization in proteomics. This bottom-up analysis requires digestion of proteins into peptides before liquid chromatography– or capillary zone electrophoresis–mass spectrometry (LC-MS or CZE-MS, respectively). Proteins are usually digested off-line using proteolytic enzymes, typically trypsin, in solution or immobilized on appropriate supports. As an alternative, here we describe on-line immobilized enzyme microreactor capillary zone electrophoresis–mass spectrometry (IMER-CZE-MS) for a straightforward, rapid, and efficient protein digestion followed by separation, detection, and characterization of the generated peptides.
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Acknowledgments
This study was supported by the Spanish Ministry of Economy and Competitiveness (RTI2018-097411-B-I00) and the Cathedra UB Rector Francisco Buscarons Úbeda (Forensic Chemistry and Chemical Engineering). Roger Pero-Gascon acknowledges the Spanish Ministry of Education, Culture and Sport for a FPU (Formación del Profesorado Universitario) fellowship.
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Pero-Gascon, R., Pont, L., Giménez, E., Sanz-Nebot, V., Benavente, F. (2022). On-line Immobilized Enzyme Microreactor Capillary Zone Electrophoresis–Mass Spectrometry for Peptide Mapping. In: Neusüß, C., Jooß, K. (eds) Capillary Electrophoresis-Mass Spectrometry . Methods in Molecular Biology, vol 2531. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2493-7_6
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DOI: https://doi.org/10.1007/978-1-0716-2493-7_6
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