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Improved Sensitivity to Determine Antibiotic Residues in Chicken Meat by In-Line Solid-Phase Extraction Coupled to Capillary Electrophoresis–Tandem Mass Spectrometry

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Capillary Electrophoresis-Mass Spectrometry

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2531))

Abstract

Traditionally, capillary electrophoresis (CE) has been ruled out of many food safety applications, despite its inherent advantages, because its concentration sensitivity has been not high enough, mainly in relation to the monitoring of contaminants and residues, such as pesticides, veterinary medicines, environmental contaminants, toxins, etc. For this reason, researchers have proposed several strategies to overcome this limitation. So far, approaches based on chromatographic principles have been the most successful solutions. These approaches, known as in-line solid phase extraction, consist of the introduction of a small amount of stationary phase in the inlet section of the electrophoretic capillary (analyte concentrator, AC) to retain the analytes before separation takes place. In this chapter, this strategy is applied to CE coupled to tandem mass spectrometry (MS/MS) for the multiresidue detection of quinolone antibiotic residues in chicken meat. A previous sample treatment based on pressurized liquid extraction to obtain an optimum performance is also described.

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Acknowledgments

This work was supported by MCIN/AEI/10.13039/501100011033/FEDER “Una manera de hacer Europa” (Project No. RTI2018-097043-B-I00) and Spanish Network of Excellence in Sample preparation (RED2018-102522-T). Thanks to Junta de Andalucía-Programa Operativo FEDER (Project No. B-AGR-202-UGR20).

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Authors and Affiliations

  1. Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Granada, Spain

    Francisco J. Lara & A. M. García-Campaña

Authors
  1. Francisco J. Lara
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  2. A. M. García-Campaña
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Corresponding author

Correspondence to A. M. García-Campaña .

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Editors and Affiliations

  1. Aalen University, Aalen, Baden-Württemberg, Germany

    Christian Neusüß

  2. Aalen University, Aalen, Baden-Württemberg, Germany

    Kevin Jooß

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Lara, F.J., García-Campaña, A.M. (2022). Improved Sensitivity to Determine Antibiotic Residues in Chicken Meat by In-Line Solid-Phase Extraction Coupled to Capillary Electrophoresis–Tandem Mass Spectrometry. In: Neusüß, C., Jooß, K. (eds) Capillary Electrophoresis-Mass Spectrometry . Methods in Molecular Biology, vol 2531. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2493-7_15

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  • DOI: https://doi.org/10.1007/978-1-0716-2493-7_15

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2492-0

  • Online ISBN: 978-1-0716-2493-7

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