Abstract
RNA:DNA hybrids not only function in various physiological cellular processes but also represent a threat to genome integrity. The methyl-5-cytosine (m5C) marked RNA:DNA hybrids containing the m5C modified RNA strand add another layer of regulation to the R-loop structure. Here we describe the detection of m5C modifications in the context of RNA:DNA hybrids using immunostaining, and identification of specific binding partners of m5C marked RNA:DNA hybrids using pull-down method.
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Yang, H., Lan, L. (2022). Immunostaining and Protein Pull-Down of Methyl-5-Cytosine-Marked RNA:DNA Hybrids. In: Aguilera, A., Ruzov, A. (eds) R-Loops . Methods in Molecular Biology, vol 2528. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2477-7_17
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DOI: https://doi.org/10.1007/978-1-0716-2477-7_17
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Publisher Name: Humana, New York, NY
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