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Reactive Oxygen Species in Plants pp 241–257Cite as

Quantitative Analysis of Posttranslational Modifications of Plant Histones

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Part of the Methods in Molecular Biology book series (MIMB,volume 2526)

Abstract

Reshaping of the chromatin landscape under oxidative stress is of paramount importance for mounting an effective stress response. Unbiased systemic identification and quantification of histone marks is crucial for understanding the epigenetic component of plant responses to adverse environmental conditions. We describe a detailed method for isolation of plant histones and subsequent bottom-up proteomics approach for characterization of acetylation and methylation status. By performing label-free quantitative mass spectrometry analysis, relative abundances of histone marks can be statistically compared between experimental conditions.

Key words

  • Histone posttranslational modifications
  • Acetylation
  • Methylation
  • Mass spectrometry
  • Histone propionylation
  • Epigenetics
  • Arabidopsis
  • Solanum
  • Nicotiana

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Acknowledgments

GL, MF, and ZZ thank for the support from the European Regional Development Fund-Project “SINGING PLANT” (No. CZ.02.1.01/0.0/0.0/16_026/0008446).

CIISB, Instruct-CZ Centre of Instruct-ERIC EU consortium, funded by MEYS CR infrastructure project LM2018127, is gratefully acknowledged for the financial support of the measurements at the CEITEC Proteomics Core Facility.

PK is supported by the European Regional Development Fund, Project Phytophthora Research Centre Reg. No. CZ.02.1.01/0.0/0.0/15_003/0000453.

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Authors and Affiliations

  1. Mendel Centre for Plant Genomics and Proteomics, Central European Institute of Technology, Masaryk University, Brno, Czech Republic

    Hana Kuchaříková, Zbyněk Zdráhal, Miloslava Fojtová & Gabriela Lochmanová

  2. Phytophthora Research Centre, Department of Molecular Biology and Radiobiology, Faculty of AgriSciences, Mendel University in Brno, Brno, Czech Republic

    Zuzana Plšková & Pavel Kerchev

Authors
  1. Hana Kuchaříková
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  2. Zuzana Plšková
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  3. Zbyněk Zdráhal
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  4. Miloslava Fojtová
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  5. Pavel Kerchev
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  6. Gabriela Lochmanová
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Corresponding author

Correspondence to Gabriela Lochmanová .

Editor information

Editors and Affiliations

  1. VIB Center for Plant Systems Biology, Ghent University, Zwijnaarde, Belgium

    Amna Mhamdi

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Kuchaříková, H., Plšková, Z., Zdráhal, Z., Fojtová, M., Kerchev, P., Lochmanová, G. (2022). Quantitative Analysis of Posttranslational Modifications of Plant Histones. In: Mhamdi, A. (eds) Reactive Oxygen Species in Plants. Methods in Molecular Biology, vol 2526. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2469-2_18

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  • DOI: https://doi.org/10.1007/978-1-0716-2469-2_18

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2468-5

  • Online ISBN: 978-1-0716-2469-2

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