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Chemical-Induced Premature Chromosome Condensation Protocol

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Chromosome Analysis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2519))

Abstract

Chromosome analysis is one of most fundamental techniques for cytogenetic studies. Chromosomes are conventionally prepared from mitotic cells arrested by colcemid block protocol. Premature chromosome condensation (PCC) technique is an alternative to obtain chromosomes. It was more than half century ago that the first observation of PCC phenomena reported. Since then, cell-fusion-mediated PCC method has been developed and introduced in many fields of chromosome analysis. More than quarter century ago, novel PCC technique using chemical drug has been developed. Afterwards, this simple and efficient drug-induced PCC technique becomes a standard protocol for preparing chromosomes. Thus, it seems to be the good time to introduce PCC technique protocol for the artisans in the field of cytogenetic studies.

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Authors and Affiliations

  1. Division of Diagnostic Imaging, Department of Radiology, Japan Labour Health and Safety Organization, Tokyo Rosai Hospital, Ohta-ku, Tokyo, Japan

    Eisuke Gotoh

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  1. Eisuke Gotoh
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Correspondence to Eisuke Gotoh .

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  1. Division of Diagnostic Imaging, Department of Radiology, Japan Labour Health and Safety Organization, Tokyo Rosai Hospital, Ohta-ku, Tokyo, Japan

    Eisuke Gotoh

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Gotoh, E. (2023). Chemical-Induced Premature Chromosome Condensation Protocol. In: Gotoh, E. (eds) Chromosome Analysis. Methods in Molecular Biology, vol 2519. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2433-3_5

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  • DOI: https://doi.org/10.1007/978-1-0716-2433-3_5

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2432-6

  • Online ISBN: 978-1-0716-2433-3

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