Abstract
Plant DNA preserved in ancient specimens has recently gained importance as a tool in comparative genomics, allowing the investigation of evolutionary processes in plant genomes through time. However, recovering the genomic information contained in such specimens is challenging owing to the presence of secondary substances that limit DNA retrieval. In this chapter, we provide a DNA extraction protocol optimized for the recovery of DNA from degraded plant materials. The protocol is based on a commercially available DNA extraction kit that does not require handling of hazardous reagents.
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Acknowledgments
O.E. was supported during his PhD by the Administrative Department of Science, Technology and Innovation of Colombia (COLCIENCIAS), grants CF14-0461/CF15-0672/CF16-0551: Recovery of lost genetic diversity in barley from the Carlsberg Foundation; and grant LP130100648: Identifying the diversity and evolution of loci associated with adaptation to aridity/heat and salinity in ancient cereal crops from the Australian Research Council.
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Estrada, O., Richards, S.M., Breen, J. (2022). Discovering the Secrets of Ancient Plants: Recovery of DNA from Museum and Archaeological Plant Specimens. In: Pereira-Santana, A., Gamboa-Tuz, S.D., Rodríguez-Zapata, L.C. (eds) Plant Comparative Genomics. Methods in Molecular Biology, vol 2512. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2429-6_15
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DOI: https://doi.org/10.1007/978-1-0716-2429-6_15
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