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Neuronal Cell Death pp 89–97Cite as

Modeling Axonal Degeneration Using Motor Nerve Organoids

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2515))

Abstract

Degeneration of axons is characteristic of many devastating diseases including amyotrophic lateral sclerosis (ALS). However, lack of an in vitro neuronal culture system that mimics damages on nerves and axonal tracts hampered development of effective treatments. Here, we describe a method to model degeneration of motor neuron axons using motor nerve organoids that are formed with human induced pluripotent stem cells. In this protocol, motor neuron axon degeneration can be rapidly induced with chemical damages. Neuroprotective effects of compounds can be examined using the degenerated axons. This motor neuron axon bundle degeneration model should facilitate future screening for drugs against diseases affecting axon fascicles.

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Acknowledgments

We thank Drs. Tatsuya Osaki, Jiro Kawada, and Teruo Fujii for critical discussion and insightful inputs on the manuscript. We also thank all members of Ikeuchi lab and Fujii lab for comments and discussion. This work was supported in part by WINGS-LST (A.C.), the Japan Society for the Promotion of Science (JSPS) with a Grant-in-Aid for a Challenging Research (Pioneering) (20 K20643), the Grant-in-Aid for Transformative Research Areas (B) (20H05786), AMED-CREST, AMED (JP20gm1410001), and Institute for AI and Beyond (Y.I.). The test of edaravone was performed in collaboration with Tanabe Mitsubishi Pharma Company.

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Author notes

  1. Shohei Kaneda

    Present address: Department of Mechanical Systems Engineering, Faculty of Engineering, Kogakuin University, Tokyo, Japan

Authors and Affiliations

  1. Institute of Industrial Science, The University of Tokyo, Tokyo, Japan

    Siu Yu A. Chow, Yui Nakanishi, Shohei Kaneda & Yoshiho Ikeuchi

  2. Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, Tokyo, Japan

    Siu Yu A. Chow, Yui Nakanishi & Yoshiho Ikeuchi

  3. Institute for AI and Beyond, The University of Tokyo, Tokyo, Japan

    Yoshiho Ikeuchi

Authors
  1. Siu Yu A. Chow
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  2. Yui Nakanishi
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  3. Shohei Kaneda
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  4. Yoshiho Ikeuchi
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Corresponding author

Correspondence to Yoshiho Ikeuchi .

Editor information

Editors and Affiliations

  1. Faculty of Medicine, University of Ottawa, Ottawa, ON, Canada

    Arezu Jahani-Asl

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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Chow, S.Y.A., Nakanishi, Y., Kaneda, S., Ikeuchi, Y. (2022). Modeling Axonal Degeneration Using Motor Nerve Organoids. In: Jahani-Asl, A. (eds) Neuronal Cell Death. Methods in Molecular Biology, vol 2515. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2409-8_6

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  • DOI: https://doi.org/10.1007/978-1-0716-2409-8_6

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2408-1

  • Online ISBN: 978-1-0716-2409-8

  • eBook Packages: Springer Protocols

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