Abstract
Suspension cell lines grow free-floating in the cell culture media without any attachment to the culture plate/vessel. Suspension cells typically mimic cells that exist in the circulation of multicellular animals such as mouse and humans. Generally, cell lines derived from the blood such as lymphocytes, megakaryocyte, and neutrophils grow in suspension. These cell lines can be used for experimental studies to understand the biology/biochemistry of cancer cells. In this chapter, procedures for working with suspension cell lines are provided, including protocols for thawing, culturing, and cryopreserving cancer cell lines. Importantly, this chapter demonstrates the best practices required to work with suspension cell lines, to minimize the risk of contaminations from adventitious microorganisms or from other cell lines.
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Acknowledgments
SLC is a senior scientist with the Beatrice Hunter Cancer Research Institute. This work was funded by the Natural Sciences and Engineering Research Council of Canada and the Janeway Children’s Hospital Foundation. MNL was supported by a Memorial University School of Graduate studies fellowship and a Cancer Research Training program award from the Beatrice Hunter Cancer Research Institute with funds provided by GIVETOLIVE Cancer Studentship. We thank Dr. Sukhinder Cheema, Peter Isesele, and Abeer A. Awad for the help with the cell micrographs and Willow R. B. Squires for helpful comments on the text.
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Longjohn, M.N., Phan, HD., Christian, S.L. (2022). Culturing Suspension Cancer Cell Lines. In: Christian, S.L. (eds) Cancer Cell Biology. Methods in Molecular Biology, vol 2508. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2376-3_2
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DOI: https://doi.org/10.1007/978-1-0716-2376-3_2
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Publisher Name: Humana, New York, NY
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