Abstract
The plant Catharanthus roseus is well known for its spatial separation of iridoid and monoterpenoid indole alkaloid (MIA) biosynthesis at both intracellular and intercellular levels, collectively suggested by RNA in situ hybridization, enzymatic and transcriptomic studies using leaf epidermis, and fluorescent protein tagging studies. Although documented in other plant species, the long-distance transport of iridoid glycosides, such as secologanin, has not been known in C. roseus until a recent study suggested that secologanin is transported from root to shoot, by grafting low iridoid/MIA mutant scions onto wild-type stock plants. This chapter describes the in vitro cultivation of C. roseus plants and grafting techniques to enable studies concerning iridoid/MIA transport between organs. The iridoid and MIA analysis methods are also provided.
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Acknowledgment
This work was supported by an NSERC Discovery Grant to YQ and an NSERC Discovery Grant to VDL. We thank Dr. Alan Blowers and colleagues from Ball Horticultural Company for preparing the M2-mutant seeds. YQ is supported by a Cannabis Health Research Chair (New Brunswick Health Research Foundation/Tetra Bio Pharma Inc.). VDL is supported by an NSERC Tier 1 Canada Research Chair in Plant Biotechnology.
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Farzana, M., Shahsavarani, M., De Luca, V., Qu, Y. (2022). Studying Iridoid Transport in Catharanthus roseus by Grafting. In: Courdavault, V., Besseau, S. (eds) Catharanthus roseus. Methods in Molecular Biology, vol 2505. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2349-7_5
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DOI: https://doi.org/10.1007/978-1-0716-2349-7_5
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