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Nuclear Pore Complex Assembly Using Xenopus Egg Extract

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The Nuclear Pore Complex

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2502))

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Abstract

Xenopus egg extract is a powerful tool for the in vitro investigation of complex cellular mechanisms. Here we describe how to obtain and employ interphase Xenopus egg extract to study nuclear pore complex assembly and how to analyze the process using Western blot or immunofluorescence based assays. The function of proteins can be conveniently assayed by high-efficient antibody mediated depletion.

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Correspondence to Wolfram Antonin .

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Holzer, G., Antonin, W. (2022). Nuclear Pore Complex Assembly Using Xenopus Egg Extract. In: Goldberg, M.W. (eds) The Nuclear Pore Complex. Methods in Molecular Biology, vol 2502. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2337-4_3

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  • DOI: https://doi.org/10.1007/978-1-0716-2337-4_3

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2336-7

  • Online ISBN: 978-1-0716-2337-4

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