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African Swine Fever Virus Hemadsorption Inhibition Assay

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African Swine Fever Virus

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2503))

Abstract

Adsorption of red blood cells (hemadsorption) on the surface of macrophages infected with African swine fever virus (ASFV) is a unique phenomenon allowing to determine virus infectious titer in hemadsorption unit (HAU) and differentiate virus strains phenotypically. In the meantime, hemadsorption of particular ASFV strain can by inhibited by homologous anti-ASFV serum containing antibody to the serogroup-specific virus protein (CD2v). Here, we describe a hemadsorption inhibition assay (HADIA) to phenotype ASFV strains to one of the known nine serogroups using blood-derived swine macrophages. The HADIA is a powerful method in the ASFV immunopathology and vaccine research since it provides additional antigenic and phenotypic characteristics of virus strains that can’t be defined by other assays.

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Acknowledgments

We appreciate Sergey Yurkov help and technical guidance for pig bone marrow cell culture preparation. We thank Yuriy Morgunov for useful discussion and assistance in HADIA and ASFV titration. We also thank Galina Koltsova for HADIA optimisation and valuable contributions.

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Authors and Affiliations

  1. Federal Research Center for Virology and Microbiology (FRCVM), Volginskiy, Russia

    Alexander Malogolovkin & Alexey Sereda

Authors
  1. Alexander Malogolovkin
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  2. Alexey Sereda
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Corresponding author

Correspondence to Alexander Malogolovkin .

Editor information

Editors and Affiliations

  1. African Swine Fever Vaccinology Group, The Pirbright Institute, Woking, UK

    Christopher L. Netherton

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Malogolovkin, A., Sereda, A. (2022). African Swine Fever Virus Hemadsorption Inhibition Assay. In: Netherton, C.L. (eds) African Swine Fever Virus. Methods in Molecular Biology, vol 2503. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2333-6_11

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  • DOI: https://doi.org/10.1007/978-1-0716-2333-6_11

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2332-9

  • Online ISBN: 978-1-0716-2333-6

  • eBook Packages: Springer Protocols

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