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Crystallization of Microbial Rhodopsins

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Rhodopsin

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2501))

Abstract

Microbial rhodopsins are light-sensitive transmembrane proteins, evolutionary adapted by various organisms like archaea, bacteria, simple eukaryote, and viruses to utilize solar energy for their survival. A complete understanding of functional mechanisms of these proteins is not possible without the knowledge of their high-resolution structures, which can be primarily obtained by X-ray crystallography. This technique, however, requires high-quality crystals, growing of which is a great challenge especially in case of membrane proteins. In this chapter, we summarize methods applied for crystallization of microbial rhodopsins with the emphasis on crystallization in lipidic mesophases, also known as in meso approach. In particular, we describe in detail the methods of crystallization using lipidic cubic phase to grow both large crystals optimized for traditional crystallographic data collection and microcrystals for serial crystallography.

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Acknowledgment

This work was supported by Russian Science Foundation (RSF) Project 21-64-00018.

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Authors and Affiliations

  1. Institut de Biologie Structurale (IBS), Université Grenoble Alpes, CEA, CNRS, Grenoble, France

    Kirill Kovalev, Roman Astashkin & Valentin Gordeliy

  2. Institute of Biological Information Processing (IBI-7: Structural Biochemistry), Forschungszentrum Jülich GmbH, Jülich, Germany

    Kirill Kovalev

  3. JuStruct: Jülich Center for Structural Biology, Forschungszentrum Jülich GmbH, Jülich, Germany

    Kirill Kovalev

  4. Research Center for Molecular Mechanisms of Aging and Age-Related Diseases, Moscow Institute of Physics and Technology (National Research University), Dolgoprudny, Russia

    Kirill Kovalev

  5. Institute of Crystallography, University of Aachen (RWTH), Aachen, Germany

    Kirill Kovalev

  6. Bridge Institute, Department of Chemistry, University of Southern California, Los Angeles, CA, USA

    Vadim Cherezov

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  1. Kirill Kovalev
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  2. Roman Astashkin
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  3. Valentin Gordeliy
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  4. Vadim Cherezov
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Correspondence to Vadim Cherezov .

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  1. Institut de Biologie Structurale (IBS), Université Grenoble Alpes, CEA, CNRS, Grenoble, France

    Valentin Gordeliy

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Kovalev, K., Astashkin, R., Gordeliy, V., Cherezov, V. (2022). Crystallization of Microbial Rhodopsins. In: Gordeliy, V. (eds) Rhodopsin. Methods in Molecular Biology, vol 2501. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2329-9_6

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  • DOI: https://doi.org/10.1007/978-1-0716-2329-9_6

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