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Rhodopsin pp 277–288Cite as

Electrophysiological Characterization of Microbial Rhodopsins by Patch-Clamp Experiments

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Part of the Methods in Molecular Biology book series (MIMB,volume 2501)

Abstract

Optogenetics is of key importance for progress in basic neuroscience research and the development of innovative future medical treatments. In particular, the use of microbial rhodopsins enables remote control of excitable-cell activity by light. The electrophysiological characterization of microbial rhodopsins is inevitable for the development of variants, which further advance optogenetic applications. Therefore, we provide a detailed description of the application of the patch-clamp method for the electrophysiological characterization of microbial rhodopsins. Here we describe the investigation of light sensitivity, wavelength- and voltage-dependence, photocurrent inactivation, kinetics, and ion selectivity.

Key words

  • Optogenetics
  • Microbial rhodopsins
  • Channelrhodopsins
  • Light-driven ion pumps
  • Patch-clamp method
  • Electrophysiological characterization

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  • DOI: 10.1007/978-1-0716-2329-9_13
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Acknowledgments

This work was supported by the Cluster of Excellence “Multiscale Bioimaging: from Molecular Machines to Networks of Excitable Cells” (MBExC), University of Göttingen, Germany.

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Correspondence to Thomas Mager .

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Mager, T. (2022). Electrophysiological Characterization of Microbial Rhodopsins by Patch-Clamp Experiments. In: Gordeliy, V. (eds) Rhodopsin. Methods in Molecular Biology, vol 2501. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2329-9_13

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  • DOI: https://doi.org/10.1007/978-1-0716-2329-9_13

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2328-2

  • Online ISBN: 978-1-0716-2329-9

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