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A Dynamic, In Vitro BBB Model to Study the Effects of Varying Levels of Shear Stress

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The Blood-Brain Barrier

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2492))

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Abstract

The blood–brain barrier (BBB) consists of a tight network of blood capillaries in the brain that separate the circulatory system from the central nervous system. Its particular properties are based on the dynamic interaction between cerebral endothelial cells and other surrounding cells, especially astrocytes. We have designed and synthesized a three-dimensional scaffold that recapitulates the main hallmarks of the BBB extracellular matrix and serves as a platform to co-culture human brain microvascular endothelial cells and human cortical astrocytes. The scaffold can be exposed to flow, thereby allowing the study of flow-mediated pathways at the BBB.

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References

  1. Abbott NJ (2013) Blood-brain barrier structure and function and the challenges for CNS drug delivery. J Inherit Metab Dis 36:437–449. https://doi.org/10.1007/s10545-013-9608-0

    Article  CAS  PubMed  Google Scholar 

  2. Garcia-Polite F, Martorell J, Del Rey-Puech P et al (2017) Pulsatility and high shear stress deteriorate barrier phenotype in brain microvascular endothelium. J Cereb Blood Flow Metab 37(7):2614–2625. https://doi.org/10.1177/0271678X16672482

    Article  CAS  PubMed  Google Scholar 

  3. Abbott NJ (2002) Astrocyte-endothelial interactions and blood-brain barrier permeability. J Anat 200:629–638. https://doi.org/10.1046/j.1469-7580.2002.00064.x

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Singer J, Trollor JN, Crawford J et al (2013) The association between pulse wave velocity and cognitive function: the Sydney memory and ageing study. PLoS One 8:4–9. https://doi.org/10.1371/journal.pone.0061855

    Article  CAS  Google Scholar 

  5. Weiss N, Miller F, Cazaubon S et al (2009) The blood-brain barrier in brain homeostasis and neurological diseases. Biochim Biophys Acta Biomembr 1788:842–857. https://doi.org/10.1016/j.bbamem.2008.10.022

    Article  CAS  Google Scholar 

  6. Nicolazzo JA, Charman SA, Charman WN (2006) Methods to assess drug permeability across the blood-brain barrier. J Pharm Pharmacol 58:281–293. https://doi.org/10.1211/jpp.58.3.0001

    Article  CAS  PubMed  Google Scholar 

  7. Bicker J, Alves G, Fortuna A et al (2014) Blood–brain barrier models and their relevance for a successful development of CNS drug delivery systems: a review. Eur J Pharm Biopharm 87:409–432. https://doi.org/10.1016/j.ejpb.2014.03.012

    Article  CAS  PubMed  Google Scholar 

  8. Cucullo L, Hossain M, Puvenna V et al (2011) The role of shear stress in blood-brain barrier endothelial physiology. BMC Neurosci 12:40. https://doi.org/10.1186/1471-2202-12-40

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Acknowledgements

This work was supported in part by a grant from Spain Ministerio de Economía y Competitividad (SAF-2017-84773-C2, MB) and by the Global CoCreation Lab (GM, MB).

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Correspondence to Jordi Martorell .

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Molins Gutiérrez, G., Martorell, J., Salazar-Martin, A.G., Balcells, M. (2022). A Dynamic, In Vitro BBB Model to Study the Effects of Varying Levels of Shear Stress. In: Stone, N. (eds) The Blood-Brain Barrier. Methods in Molecular Biology, vol 2492. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2289-6_10

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  • DOI: https://doi.org/10.1007/978-1-0716-2289-6_10

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2288-9

  • Online ISBN: 978-1-0716-2289-6

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