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Epiblast Stem Cells pp 235–249Cite as

Differentiation of Human-Induced Pluripotent Stem Cells (hiPSCs) into Human Primordial Germ Cell-like Cells (hPGCLCs) In Vitro

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Part of the Methods in Molecular Biology book series (MIMB,volume 2490)

Abstract

In humans, germ cells are specified in the extraembryonic yolk sac, at proximity of allantois, around the second week of gestation. Derivation of human germ cell-like cells (hPGCLCs) from human pluripotent cells in vitro is of a great importance for research purposes, such as disease modeling, or studying the early human germ cell development and the effect of environmental factors on this development. As it is not possible to access human embryos at early developmental stages, a two-step protocol has been proposed by Sasaki and colleagues to differentiate hPGCLCs in vitro from human pluripotent stem cells. Here, we report a detailed protocol for in vitro hPGCLCs differentiation from induced pluripotent stem cells (iPSCs).

Key words

  • Germ cell specification
  • hiPSCs
  • hiMeLCs
  • hPGCLCs

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  • DOI: 10.1007/978-1-0716-2281-0_17
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Acknowledgements

This work was supported by Åke Wibergs Foundation, Jeanssons Foundation, Swedish Research Council (Vetenskapsradet, 2018-02557), Birgitta and Carl-Axel Rydbeck research foundation, and Karolinska Institutet to Qiaolin Deng.

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Authors and Affiliations

  1. Department of Physiology and Pharmacology, Karolinska Institutet, Solna, Sweden

    Ahmed Reda & Qiaolin Deng

  2. NORDFERTIL Research Lab Stockholm, Childhood Cancer Research Unit, Department of Women’s and Children’s Health, Karolinska Institutet, and Karolinska University Hospital, Solna, Sweden

    Jan-Bernd Stukenborg

  3. Center for Molecular Medicine, Karolinska University Hospital, Solna, Sweden

    Qiaolin Deng

Authors
  1. Ahmed Reda
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  2. Jan-Bernd Stukenborg
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  3. Qiaolin Deng
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Corresponding author

Correspondence to Qiaolin Deng .

Editor information

Editors and Affiliations

  1. Swiss Cancer Research Institute, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland

    Pierre Osteil

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Reda, A., Stukenborg, JB., Deng, Q. (2022). Differentiation of Human-Induced Pluripotent Stem Cells (hiPSCs) into Human Primordial Germ Cell-like Cells (hPGCLCs) In Vitro. In: Osteil, P. (eds) Epiblast Stem Cells. Methods in Molecular Biology, vol 2490. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2281-0_17

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  • DOI: https://doi.org/10.1007/978-1-0716-2281-0_17

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2280-3

  • Online ISBN: 978-1-0716-2281-0

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