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Epiblast Stem Cells pp 213–233Cite as

In Vitro Differentiation of Murine Embryonic Stem Cells (ESCs) into Primordial Germ Cell-like Cells (PGCLCs)

Part of the Methods in Molecular Biology book series (MIMB,volume 2490)

Abstract

The ability to generate primordial germ cell-like cells (PGCLCs) from murine embryonic stem cells (ESCs) has enabled in vitro investigation of the molecular mechanisms regulating this process without the use of a mouse model. Here we describe the procedures from the culture of ESCs to the detection of PGCLCs in the embryoid bodies (spheroids).

Key words

  • Primordial germ cell-like cells
  • Epiblast-like cells
  • Embryonic stem cells
  • In vitro differentiation

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  • DOI: 10.1007/978-1-0716-2281-0_16
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Acknowledgements

We thank Hiroshi Ohta and Mitinori Saitou for providing us BDF1-2 ES cells. We also thank Jaromir Mikes for flow cytometry. This work was supported by Åke Wibergs Foundation, Jeanssons Foundation, Swedish Research Council (Vetenskapsradet, 2018-02557), Birgitta and Carl-Axel Rydbeck research foundation, and Karolinska Institutet to Q.D.

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Authors and Affiliations

  1. Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden

    Han-Pin Pui & Qiaolin Deng

  2. Center for Molecular Medicine, Karolinska University Hospital, Stockholm, Sweden

    Han-Pin Pui & Qiaolin Deng

Authors
  1. Han-Pin Pui
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  2. Qiaolin Deng
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Corresponding author

Correspondence to Qiaolin Deng .

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Editors and Affiliations

  1. Swiss Cancer Research Institute, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland

    Pierre Osteil

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Pui, HP., Deng, Q. (2022). In Vitro Differentiation of Murine Embryonic Stem Cells (ESCs) into Primordial Germ Cell-like Cells (PGCLCs). In: Osteil, P. (eds) Epiblast Stem Cells. Methods in Molecular Biology, vol 2490. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2281-0_16

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  • DOI: https://doi.org/10.1007/978-1-0716-2281-0_16

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