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Multienzymatic Assemblies pp 361–375Cite as

Enzymatic Photometric Assays for the Selective Detection of Halides

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2487))

Abstract

Halides are substrates and products of a number of biotechnologically important enzymes like dehalogenases, halide methyltransferases, and halogenases. Therefore, the determination of halide concentrations in samples is important. The classical methods based on mercuric thiocyanate are very dangerous, produce hazardous waste, and do not discriminate between chloride, bromide, and iodide. In this chapter, we describe a detailed protocol for the determination of halide concentrations based on the haloperoxidase-catalyzed oxidation of halides. The resulting hypohalous acids are detected using commercially available colorimetric or fluorimetric probes. The biocatalytic nature of the assays allows them to be implemented in one-pot cascade reactions with halide-generating enzymes. Since chloride is ubiquitous in biological systems, we also describe convenient photometric assays for the selective detection of bromide and iodide in the presence of chloride, obviating the need for laborious dialyses to obtain halide-free enzymes and reagents.

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Acknowledgement

Q. T. thanks the China Scholarship Council for financial support of her PhD thesis project (File No.: 201606150073). A. S. A.-Ü. and E. D. S. thank the European Union (722610 ES-CAT).

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Authors and Affiliations

  1. Institute of Biochemistry, University of Greifswald, Greifswald, Germany

    Qingyun Tang, Askin S. Aslan-Üzel, Eva D. Schuiten, Christoffel P. S. Badenhorst & Uwe T. Bornscheuer

  2. Department of Chemistry, University of Crete, Heraklion, Greece

    Ioannis V. Pavlidis

Authors
  1. Qingyun Tang
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  2. Askin S. Aslan-Üzel
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  3. Eva D. Schuiten
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  4. Christoffel P. S. Badenhorst
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  5. Ioannis V. Pavlidis
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  6. Uwe T. Bornscheuer
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Corresponding authors

Correspondence to Christoffel P. S. Badenhorst , Ioannis V. Pavlidis or Uwe T. Bornscheuer .

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Editors and Affiliations

  1. Department of Biological Applications and Technologies, University of Ioannina, Ioannina, Greece

    Haralambos Stamatis

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Tang, Q., Aslan-Üzel, A.S., Schuiten, E.D., Badenhorst, C.P.S., Pavlidis, I.V., Bornscheuer, U.T. (2022). Enzymatic Photometric Assays for the Selective Detection of Halides. In: Stamatis, H. (eds) Multienzymatic Assemblies. Methods in Molecular Biology, vol 2487. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2269-8_22

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  • DOI: https://doi.org/10.1007/978-1-0716-2269-8_22

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2268-1

  • Online ISBN: 978-1-0716-2269-8

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