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In Vivo Imaging of Circadian NET Formation During Lung Injury by Four-Dimensional Intravital Microscopy

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Circadian Regulation

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2482))

Abstract

Neutrophil extracellular traps (NETs) are toxic extracellular structures deployed by neutrophils in response to pathogens and sterile danger signals. NETs are circadian in nature as mouse and human neutrophils preferentially deploy them at night or early morning. Traditionally, NETs have been quantified using a plethora of methods including immunofluorescence and ELISA-based assays; however few options are available to visualize them in vivo. Here we describe a method to directly visualize and quantify NET formation and release in the microvasculature of the lung using intravital imaging in a model of acute lung injury. The method allows four-dimensional capture and quantification of NET formation dynamics over time and should be a useful resource for those interested in visualizing neutrophil responses in vivo.

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Author information

Authors and Affiliations

  1. Area of Cell and Developmental Biology, Fundación Centro Nacional de Investigaciones Cardiovasculares (CNIC), Madrid, Spain

    Alejandra Aroca-Crevillén, Andres Hidalgo & Jose M. Adrover

  2. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, USA

    Jose M. Adrover

Authors
  1. Alejandra Aroca-Crevillén
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  2. Andres Hidalgo
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  3. Jose M. Adrover
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Corresponding author

Correspondence to Jose M. Adrover .

Editor information

Editors and Affiliations

  1. Institute for Research in Biomedicine, Barcelona Institute for Science and Tech, Barcelona, Spain

    Guiomar Solanas

  2. Hospital del Mar Medical Research Institute (IMIM), Barcelona, Spain

    Patrick -Simon Welz

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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Aroca-Crevillén, A., Hidalgo, A., Adrover, J.M. (2022). In Vivo Imaging of Circadian NET Formation During Lung Injury by Four-Dimensional Intravital Microscopy. In: Solanas, G., Welz, P.S. (eds) Circadian Regulation. Methods in Molecular Biology, vol 2482. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2249-0_19

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  • DOI: https://doi.org/10.1007/978-1-0716-2249-0_19

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2248-3

  • Online ISBN: 978-1-0716-2249-0

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