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Malaria Immunology pp 457–466Cite as

Assessing Antigen Presentation on the Surface of Plasmodium falciparum-Infected Erythrocytes by Photoactivated Localization Microscopy (PALM)

Part of the Methods in Molecular Biology book series (MIMB,volume 2470)

Abstract

Super-resolution microscopy in the form of photoactivated localization microscopy (PALM) offers the possibility of counting single molecules in a cell, a cellular compartment or a molecular complex. PALM can, therefore, underpin molecular and biochemical processes with a numeric and stoichiometric understanding of the interacting players. Here, we introduce the physical principles underlying PALM and provide a step-by-step protocol of how to apply PALM to questions related to the biology and pathophysiology of P. falciparum and other malaria parasites.

Key words

  • Single-molecule counting
  • Photoactivated localization microscopy
  • PALM
  • PfEMP1
  • STORM

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Acknowledgments

This work was funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation)—Project number 240245660—SFB 1129 (M.L.) and SFB 807 (C.K. and M.H.)

Author information

Authors and Affiliations

  1. Institute for Physical and Theoretical Chemistry, Goethe-University Frankfurt, Frankfurt, Germany

    Christos Karathanasis & Mike Heilemann

  2. Center of Infectious Diseases, Parasitology, Universitätsklinikum Heidelberg, Heidelberg, Germany

    Cecilia P. Sanchez & Michael Lanzer

  3. BioQuant-Center for Quantitative Biology, Heidelberg University, Heidelberg, Germany

    Mike Heilemann

Authors
  1. Christos Karathanasis
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  2. Cecilia P. Sanchez
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  3. Mike Heilemann
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  4. Michael Lanzer
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Corresponding author

Correspondence to Michael Lanzer .

Editor information

Editors and Affiliations

  1. Centre for Medical Parasitology, Department of Immunology and Microbiology, University of Copenhagen, Copenhagen, Denmark

    Prof. Anja Tatiana Ramstedt Jensen

  2. Centre for Medical Parasitology, Department of Immunology and Microbiology, University of Copenhagen, Copenhagen, Denmark

    Prof. Lars Hviid

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Karathanasis, C., Sanchez, C.P., Heilemann, M., Lanzer, M. (2022). Assessing Antigen Presentation on the Surface of Plasmodium falciparum-Infected Erythrocytes by Photoactivated Localization Microscopy (PALM). In: Jensen, A.T.R., Hviid, L. (eds) Malaria Immunology. Methods in Molecular Biology, vol 2470. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2189-9_34

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  • DOI: https://doi.org/10.1007/978-1-0716-2189-9_34

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2188-2

  • Online ISBN: 978-1-0716-2189-9

  • eBook Packages: Springer Protocols

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