Immuno-electron microscopy can detect and localize antigens in cells or tissues at a resolution of several nanometers. In the case of P. falciparum-infected erythrocytes, immuno-EM studies are frequently hampered by the electron-dense nature of the hemoglobin and access of antibodies to antigenic sites, particularly if the targeted protein is presented on the host cell surface or lies in proximity to the host cell cytoskeleton. Here, we describe an improved immuno-EM protocol that overcomes these problems. The improved signal to noise ratio and the enhanced access to antigenic sites now allows one to obtain information regarding target density and distribution and, hence, additional insights into the architecture and function of parasite-induced, or -affected, structures.
- Antigen accessibility
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This work was funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation)—Project number 240245660—SFB 1129 (M.L. and U.S.S.).
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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
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Cyrklaff, M., Sanchez, C.P., Hanebutte, L., Jäger, J., Schwarz, U.S., Lanzer, M. (2022). An Improved Method for Assessing Antigen Presentation on the Surface of Plasmodium falciparum-Infected Erythrocytes by Immuno-Electron Microscopy. In: Jensen, A.T.R., Hviid, L. (eds) Malaria Immunology. Methods in Molecular Biology, vol 2470. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2189-9_33
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-2188-2
Online ISBN: 978-1-0716-2189-9
eBook Packages: Springer Protocols