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Extraction and Analysis of Phenolic Compounds from Grape Berries

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Plant Secondary Metabolism Engineering

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2469))

Abstract

Phenolics are ubiquitous compounds that represent the most abundant and diverse class of plant metabolites. From an analytical point of view, phenolics can be divided into soluble phenolics such as phenolic acids, phenylpropanoids, flavonoids and quinones, and nonsoluble compounds such as proanthocyanidins, lignins, and cell wall–bound hydroxycinnamic acids. Extraction of phenolics from the sample material is the first step toward their analysis. Biochemical methods for determination of total phenolics content were widely used in the past but modern chromatographic and mass spectrometric methods for identification and quantification of individual compounds are available in recent years. In this chapter, we describe methods for phenolic compounds extraction used in our laboratories from berries of Vitis vinifera and analytical methods including HPLC coupled to DAD detector and Q-TOF LC/MS for their analysis.

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Acknowledgments

This work was funded by a United States-Israel Binational Agricultural Research and Development (BARD) fund grant (No. IS-4957-16R).

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Authors and Affiliations

  1. Department of Postharvest Science, Agricultural Research Organization, The Volcani Center, Rishon LeZion, Israel

    Kamal Tyagi, Itay Maoz & Amnon Lichter

  2. Department of Viticulture and Enology, University of California, Davis, CA, USA

    Kamal Tyagi, Larry Lerno & Susan E. Ebeler

  3. Food Safety and Measurement Facility, University of California, Davis, CA, USA

    Larry Lerno

  4. Chemistry Lab., Council for Agricultural Research and Economics – Viticulture & Enology (CREA-VE), Conegliano, Italy

    Mirko De Rosso & Riccardo Flamini

Authors
  1. Kamal Tyagi
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  2. Larry Lerno
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  3. Mirko De Rosso
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  4. Itay Maoz
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  5. Amnon Lichter
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  6. Susan E. Ebeler
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  7. Riccardo Flamini
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Corresponding authors

Correspondence to Susan E. Ebeler or Riccardo Flamini .

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Editors and Affiliations

  1. Center for Biotechnology and Department of Botany, Plant Physiology Laboratory, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, Brazil

    Arthur Germano Fett-Neto

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Tyagi, K. et al. (2022). Extraction and Analysis of Phenolic Compounds from Grape Berries. In: Fett-Neto, A.G. (eds) Plant Secondary Metabolism Engineering. Methods in Molecular Biology, vol 2469. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2185-1_1

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  • DOI: https://doi.org/10.1007/978-1-0716-2185-1_1

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2184-4

  • Online ISBN: 978-1-0716-2185-1

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